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        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2023-04-25, 20:06 CEST based on data in:

        General Statistics

        Showing 494/494 rows and 3/6 columns.
        Sample Name% Dups% GCM Seqs
        DRR127488_1
        47.6%
        46%
        41.1
        DRR127488_2
        47.2%
        46%
        41.1
        DRR127524_1
        50.2%
        47%
        38.2
        DRR127524_2
        49.9%
        46%
        38.2
        DRR127532_1
        36.2%
        45%
        16.7
        DRR127532_2
        35.2%
        45%
        16.7
        DRR127535_1
        35.9%
        46%
        13.9
        DRR127535_2
        35.7%
        46%
        13.9
        DRR127537_1
        36.1%
        48%
        20.4
        DRR127537_2
        35.9%
        48%
        20.4
        DRR127546_1
        51.1%
        45%
        49.7
        DRR127546_2
        50.7%
        45%
        49.7
        DRR127552_1
        34.6%
        44%
        13.8
        DRR127552_2
        34.5%
        44%
        13.8
        DRR127583_1
        31.6%
        47%
        23.7
        DRR127583_2
        31.1%
        46%
        23.7
        DRR127588_1
        85.7%
        44%
        19.1
        DRR127588_2
        84.8%
        44%
        19.1
        DRR127596_1
        31.5%
        43%
        18.3
        DRR127596_2
        31.0%
        43%
        18.3
        DRR127597_1
        32.7%
        46%
        16.7
        DRR127597_2
        32.4%
        46%
        16.7
        DRR127613_1
        32.4%
        48%
        19.6
        DRR127613_2
        32.2%
        48%
        19.6
        DRR127616_1
        29.8%
        46%
        22.1
        DRR127616_2
        29.6%
        46%
        22.1
        DRR127619_1
        43.7%
        42%
        17.0
        DRR127619_2
        43.2%
        42%
        17.0
        DRR127628_1
        31.5%
        45%
        20.3
        DRR127628_2
        31.5%
        45%
        20.3
        DRR127634_1
        47.3%
        45%
        34.0
        DRR127634_2
        46.7%
        45%
        34.0
        DRR127649_1
        32.5%
        48%
        19.6
        DRR127649_2
        32.3%
        47%
        19.6
        DRR127672_1
        37.9%
        45%
        20.4
        DRR127672_2
        37.3%
        45%
        20.4
        DRR127683_1
        45.6%
        46%
        20.6
        DRR127683_2
        45.4%
        46%
        20.6
        DRR127692_1
        36.1%
        46%
        17.7
        DRR127692_2
        36.1%
        46%
        17.7
        DRR127704_1
        34.3%
        44%
        20.0
        DRR127704_2
        33.0%
        44%
        20.0
        DRR127707_1
        43.2%
        45%
        29.2
        DRR127707_2
        41.8%
        45%
        29.2
        DRR127713_1
        47.3%
        43%
        23.4
        DRR127713_2
        46.6%
        43%
        23.4
        DRR127721_1
        45.4%
        43%
        21.3
        DRR127721_2
        45.2%
        42%
        21.3
        DRR127724_1
        43.5%
        43%
        28.4
        DRR127724_2
        43.1%
        43%
        28.4
        DRR127728_1
        55.2%
        43%
        26.9
        DRR127728_2
        55.0%
        43%
        26.9
        DRR127731_1
        42.2%
        46%
        32.3
        DRR127731_2
        42.1%
        46%
        32.3
        DRR127736_1
        37.8%
        43%
        20.7
        DRR127736_2
        37.4%
        43%
        20.7
        DRR127737_1
        47.3%
        46%
        31.9
        DRR127737_2
        47.2%
        46%
        31.9
        DRR127748_1
        33.8%
        46%
        17.4
        DRR127748_2
        33.9%
        46%
        17.4
        DRR127751_1
        40.3%
        49%
        27.1
        DRR127751_2
        40.1%
        49%
        27.1
        DRR127752_1
        49.6%
        39%
        23.9
        DRR127752_2
        49.0%
        39%
        23.9
        DRR127755_1
        33.8%
        44%
        21.8
        DRR127755_2
        33.9%
        44%
        21.8
        DRR127756_1
        41.3%
        43%
        25.5
        DRR127756_2
        41.2%
        43%
        25.5
        DRR127762_1
        43.1%
        44%
        26.1
        DRR127762_2
        42.9%
        44%
        26.1
        DRR127763_1
        38.2%
        46%
        23.9
        DRR127763_2
        38.2%
        46%
        23.9
        DRR127776_1
        44.7%
        49%
        27.5
        DRR127776_2
        44.8%
        49%
        27.5
        DRR127777_1
        27.8%
        45%
        26.3
        DRR127777_2
        27.6%
        45%
        26.3
        DRR162775_1
        45.1%
        44%
        20.3
        DRR162775_2
        44.9%
        44%
        20.3
        DRR162776_1
        54.1%
        44%
        15.4
        DRR162776_2
        53.6%
        43%
        15.4
        DRR171467_1
        53.0%
        41%
        34.9
        DRR171467_2
        52.7%
        41%
        34.9
        DRR171469_1
        31.3%
        46%
        23.8
        DRR171469_2
        31.2%
        46%
        23.8
        DRR171474_1
        42.9%
        42%
        17.1
        DRR171474_2
        42.6%
        42%
        17.1
        DRR171477_1
        34.4%
        43%
        20.3
        DRR171477_2
        33.9%
        43%
        20.3
        DRR171479_1
        37.8%
        48%
        15.5
        DRR171479_2
        37.3%
        48%
        15.5
        DRR171487_1
        28.5%
        46%
        16.9
        DRR171487_2
        28.4%
        46%
        16.9
        DRR171488_1
        60.9%
        53%
        21.1
        DRR171488_2
        60.5%
        53%
        21.1
        DRR171497_1
        32.0%
        45%
        25.5
        DRR171497_2
        31.2%
        45%
        25.5
        DRR171499_1
        47.5%
        44%
        27.3
        DRR171499_2
        45.6%
        44%
        27.3
        DRR171500_1
        28.2%
        47%
        27.7
        DRR171500_2
        26.5%
        47%
        27.7
        DRR171503_1
        40.5%
        44%
        27.8
        DRR171503_2
        40.1%
        44%
        27.8
        DRR171506_1
        34.6%
        43%
        21.8
        DRR171506_2
        33.9%
        43%
        21.8
        DRR171509_1
        31.6%
        50%
        23.1
        DRR171509_2
        31.4%
        50%
        23.1
        DRR171513_1
        24.8%
        45%
        14.8
        DRR171513_2
        24.2%
        45%
        14.8
        DRR171514_1
        27.1%
        46%
        16.2
        DRR171514_2
        27.1%
        46%
        16.2
        DRR171515_1
        28.7%
        46%
        20.2
        DRR171515_2
        28.6%
        46%
        20.2
        DRR171517_1
        34.0%
        47%
        11.5
        DRR171517_2
        33.6%
        47%
        11.5
        DRR171518_1
        22.0%
        47%
        8.9
        DRR171518_2
        22.0%
        47%
        8.9
        DRR171523_1
        21.4%
        47%
        13.6
        DRR171523_2
        21.5%
        47%
        13.6
        DRR171525_1
        29.5%
        46%
        14.2
        DRR171525_2
        29.5%
        46%
        14.2
        DRR171527_1
        27.9%
        48%
        18.9
        DRR171527_2
        27.8%
        48%
        18.9
        DRR171530_1
        34.0%
        46%
        12.5
        DRR171530_2
        33.9%
        46%
        12.5
        DRR171538_1
        42.1%
        48%
        24.1
        DRR171538_2
        41.8%
        48%
        24.1
        DRR171539_1
        18.9%
        50%
        14.3
        DRR171539_2
        18.8%
        50%
        14.3
        DRR171543_1
        32.6%
        46%
        11.7
        DRR171543_2
        32.2%
        47%
        11.7
        DRR171545_1
        15.0%
        50%
        11.5
        DRR171545_2
        14.9%
        50%
        11.5
        DRR171546_1
        52.1%
        46%
        27.1
        DRR171546_2
        51.6%
        46%
        27.1
        DRR171552_1
        43.0%
        45%
        20.0
        DRR171552_2
        42.5%
        45%
        20.0
        DRR171555_1
        26.0%
        46%
        18.6
        DRR171555_2
        25.8%
        46%
        18.6
        DRR171560_1
        23.8%
        47%
        8.1
        DRR171560_2
        23.7%
        47%
        8.1
        DRR171563_1
        37.6%
        47%
        17.2
        DRR171563_2
        37.1%
        47%
        17.2
        DRR171567_1
        29.8%
        46%
        20.5
        DRR171567_2
        29.2%
        46%
        20.5
        DRR171568_1
        25.8%
        46%
        12.9
        DRR171568_2
        25.3%
        46%
        12.9
        DRR171569_1
        23.9%
        47%
        16.4
        DRR171569_2
        23.6%
        47%
        16.4
        DRR171571_1
        30.9%
        48%
        16.5
        DRR171571_2
        30.5%
        48%
        16.5
        DRR171572_1
        25.2%
        46%
        22.4
        DRR171572_2
        24.6%
        46%
        22.4
        DRR171576_1
        14.6%
        42%
        0.4
        DRR171576_2
        14.1%
        42%
        0.4
        DRR171578_1
        34.9%
        44%
        21.1
        DRR171578_2
        34.7%
        44%
        21.1
        DRR171580_1
        33.7%
        49%
        23.4
        DRR171580_2
        33.4%
        49%
        23.4
        DRR171581_1
        40.7%
        47%
        30.4
        DRR171581_2
        40.5%
        47%
        30.4
        DRR171585_1
        24.5%
        49%
        19.5
        DRR171585_2
        24.5%
        49%
        19.5
        DRR171586_1
        20.3%
        49%
        17.4
        DRR171586_2
        20.2%
        49%
        17.4
        DRR171587_1
        34.1%
        51%
        18.5
        DRR171587_2
        34.0%
        51%
        18.5
        DRR171588_1
        37.3%
        48%
        27.2
        DRR171588_2
        36.8%
        48%
        27.2
        DRR171589_1
        34.4%
        43%
        9.5
        DRR171589_2
        34.1%
        43%
        9.5
        DRR171591_1
        34.6%
        46%
        21.3
        DRR171591_2
        34.1%
        46%
        21.3
        DRR171592_1
        38.8%
        45%
        20.0
        DRR171592_2
        38.0%
        45%
        20.0
        DRR171594_1
        43.5%
        45%
        25.3
        DRR171594_2
        42.5%
        45%
        25.3
        DRR171598_1
        30.9%
        48%
        21.6
        DRR171598_2
        30.6%
        48%
        21.6
        DRR171599_1
        38.5%
        46%
        19.8
        DRR171599_2
        36.9%
        46%
        19.8
        DRR171601_1
        39.4%
        45%
        24.4
        DRR171601_2
        38.4%
        45%
        24.4
        DRR171604_1
        24.6%
        48%
        21.2
        DRR171604_2
        24.4%
        48%
        21.2
        DRR171605_1
        23.5%
        47%
        19.6
        DRR171605_2
        23.3%
        47%
        19.6
        DRR171606_1
        37.0%
        45%
        24.2
        DRR171606_2
        36.7%
        45%
        24.2
        DRR171607_1
        38.5%
        44%
        22.9
        DRR171607_2
        38.0%
        44%
        22.9
        DRR171608_1
        40.0%
        45%
        23.2
        DRR171608_2
        38.6%
        45%
        23.2
        DRR171610_1
        30.7%
        48%
        26.0
        DRR171610_2
        30.1%
        48%
        26.0
        DRR171613_1
        36.4%
        45%
        25.0
        DRR171613_2
        34.8%
        45%
        25.0
        DRR171616_1
        46.7%
        45%
        26.1
        DRR171616_2
        46.0%
        45%
        26.1
        DRR171617_1
        63.7%
        46%
        29.7
        DRR171617_2
        62.4%
        46%
        29.7
        DRR171619_1
        30.2%
        45%
        26.0
        DRR171619_2
        30.0%
        45%
        26.0
        DRR171620_1
        35.2%
        44%
        24.7
        DRR171620_2
        34.8%
        44%
        24.7
        DRR171621_1
        27.6%
        45%
        22.0
        DRR171621_2
        26.7%
        45%
        22.0
        DRR171623_1
        49.4%
        47%
        24.5
        DRR171623_2
        48.7%
        47%
        24.5
        DRR171624_1
        46.3%
        44%
        25.4
        DRR171624_2
        45.7%
        44%
        25.4
        DRR171625_1
        29.3%
        48%
        25.7
        DRR171625_2
        28.8%
        48%
        25.7
        DRR171626_1
        41.9%
        45%
        12.8
        DRR171626_2
        40.9%
        45%
        12.8
        DRR171628_1
        39.3%
        45%
        33.9
        DRR171628_2
        38.5%
        45%
        33.9
        DRR171629_1
        51.1%
        44%
        39.1
        DRR171629_2
        50.4%
        44%
        39.1
        DRR171631_1
        47.1%
        46%
        24.8
        DRR171631_2
        46.6%
        46%
        24.8
        DRR171637_1
        46.4%
        43%
        28.0
        DRR171637_2
        45.8%
        43%
        28.0
        DRR171639_1
        31.6%
        47%
        28.2
        DRR171639_2
        30.1%
        47%
        28.2
        DRR171640_1
        37.2%
        47%
        37.5
        DRR171640_2
        35.7%
        47%
        37.5
        DRR171641_1
        43.4%
        44%
        23.8
        DRR171641_2
        42.7%
        44%
        23.8
        DRR171642_1
        38.6%
        46%
        26.5
        DRR171642_2
        37.8%
        46%
        26.5
        DRR171643_1
        34.7%
        44%
        22.4
        DRR171643_2
        34.6%
        44%
        22.4
        DRR171644_1
        46.3%
        46%
        23.1
        DRR171644_2
        45.7%
        46%
        23.1
        DRR171645_1
        35.8%
        46%
        29.6
        DRR171645_2
        35.3%
        46%
        29.6
        DRR171646_1
        36.2%
        45%
        22.8
        DRR171646_2
        35.5%
        45%
        22.8
        DRR171647_1
        32.9%
        43%
        10.5
        DRR171647_2
        32.5%
        43%
        10.5
        DRR171648_1
        27.4%
        45%
        23.0
        DRR171648_2
        27.2%
        45%
        23.0
        DRR171650_1
        22.4%
        46%
        16.9
        DRR171650_2
        22.2%
        46%
        16.9
        DRR171651_1
        26.2%
        49%
        23.2
        DRR171651_2
        26.1%
        49%
        23.2
        DRR171652_1
        38.4%
        46%
        22.7
        DRR171652_2
        37.6%
        46%
        22.7
        DRR171653_1
        95.9%
        45%
        29.1
        DRR171653_2
        94.4%
        45%
        29.1
        DRR171654_1
        33.0%
        43%
        18.3
        DRR171654_2
        32.7%
        43%
        18.3
        DRR171655_1
        33.8%
        44%
        23.1
        DRR171655_2
        33.2%
        44%
        23.1
        DRR171656_1
        53.1%
        49%
        19.2
        DRR171656_2
        52.0%
        49%
        19.2
        DRR171657_1
        32.5%
        47%
        22.1
        DRR171657_2
        31.9%
        47%
        22.1
        DRR171659_1
        34.3%
        47%
        25.8
        DRR171659_2
        33.4%
        47%
        25.8
        DRR171662_1
        60.4%
        42%
        20.3
        DRR171662_2
        57.1%
        42%
        20.3
        DRR171663_1
        87.6%
        44%
        23.7
        DRR171663_2
        84.9%
        44%
        23.7
        DRR171673_1
        30.5%
        46%
        25.7
        DRR171673_2
        29.6%
        46%
        25.7
        DRR171676_1
        30.4%
        47%
        23.9
        DRR171676_2
        29.5%
        47%
        23.9
        DRR171686_1
        28.8%
        45%
        24.9
        DRR171686_2
        28.4%
        45%
        24.9
        DRR171689_1
        22.5%
        46%
        15.4
        DRR171689_2
        22.5%
        46%
        15.4
        DRR171691_1
        32.1%
        45%
        25.6
        DRR171691_2
        31.5%
        45%
        25.6
        DRR171694_1
        34.2%
        46%
        25.9
        DRR171694_2
        33.2%
        46%
        25.9
        DRR171698_1
        41.5%
        46%
        25.7
        DRR171698_2
        41.1%
        46%
        25.7
        DRR171700_1
        40.0%
        42%
        19.0
        DRR171700_2
        39.6%
        42%
        19.0
        DRR171705_1
        24.6%
        47%
        15.9
        DRR171705_2
        24.1%
        47%
        15.9
        DRR171710_1
        30.3%
        48%
        23.9
        DRR171710_2
        29.7%
        48%
        23.9
        DRR171711_1
        28.8%
        46%
        21.0
        DRR171711_2
        28.7%
        46%
        21.0
        DRR171712_1
        26.1%
        46%
        23.1
        DRR171712_2
        25.5%
        46%
        23.1
        DRR171713_1
        26.9%
        43%
        22.7
        DRR171713_2
        26.4%
        43%
        22.7
        DRR171714_1
        43.9%
        43%
        24.9
        DRR171714_2
        43.0%
        43%
        24.9
        DRR171715_1
        29.5%
        45%
        25.6
        DRR171715_2
        28.9%
        45%
        25.6
        DRR171716_1
        24.7%
        47%
        22.9
        DRR171716_2
        23.9%
        47%
        22.9
        DRR171717_1
        31.6%
        46%
        17.8
        DRR171717_2
        31.3%
        46%
        17.8
        DRR171718_1
        96.3%
        52%
        7.9
        DRR171718_2
        95.3%
        52%
        7.9
        DRR171719_1
        27.2%
        46%
        27.2
        DRR171719_2
        25.5%
        46%
        27.2
        DRR171720_1
        34.7%
        45%
        21.9
        DRR171720_2
        32.4%
        45%
        21.9
        DRR171721_1
        17.3%
        46%
        2.3
        DRR171721_2
        17.0%
        46%
        2.3
        DRR171722_1
        37.9%
        46%
        19.7
        DRR171722_2
        37.0%
        46%
        19.7
        DRR171723_1
        43.7%
        45%
        17.3
        DRR171723_2
        42.8%
        45%
        17.3
        DRR171724_1
        56.8%
        45%
        42.3
        DRR171724_2
        56.6%
        45%
        42.3
        DRR171725_1
        36.9%
        45%
        23.7
        DRR171725_2
        36.5%
        45%
        23.7
        DRR171726_1
        36.6%
        45%
        18.0
        DRR171726_2
        35.7%
        45%
        18.0
        DRR171727_1
        45.5%
        48%
        31.7
        DRR171727_2
        45.1%
        48%
        31.7
        DRR171733_1
        47.4%
        45%
        35.0
        DRR171733_2
        47.1%
        45%
        35.0
        DRR171737_1
        33.6%
        46%
        21.9
        DRR171737_2
        33.3%
        46%
        21.9
        DRR171741_1
        43.4%
        48%
        37.9
        DRR171741_2
        43.1%
        48%
        37.9
        DRR171745_1
        47.5%
        43%
        14.3
        DRR171745_2
        46.8%
        43%
        14.3
        DRR171747_1
        35.4%
        45%
        28.7
        DRR171747_2
        35.2%
        45%
        28.7
        DRR171755_1
        42.2%
        46%
        32.1
        DRR171755_2
        42.0%
        46%
        32.1
        DRR171762_1
        39.6%
        47%
        27.3
        DRR171762_2
        39.3%
        47%
        27.3
        DRR171763_1
        23.8%
        46%
        17.3
        DRR171763_2
        23.9%
        46%
        17.3
        DRR171765_1
        34.1%
        49%
        19.5
        DRR171765_2
        34.0%
        48%
        19.5
        DRR171770_1
        39.5%
        47%
        26.8
        DRR171770_2
        39.4%
        47%
        26.8
        DRR171771_1
        42.0%
        47%
        13.9
        DRR171771_2
        41.2%
        47%
        13.9
        DRR171772_1
        51.7%
        46%
        15.5
        DRR171772_2
        51.1%
        46%
        15.5
        DRR171773_1
        33.4%
        45%
        21.8
        DRR171773_2
        33.4%
        45%
        21.8
        DRR171777_1
        42.1%
        45%
        35.8
        DRR171777_2
        41.9%
        45%
        35.8
        DRR171779_1
        37.3%
        46%
        29.4
        DRR171779_2
        37.0%
        46%
        29.4
        DRR171782_1
        33.3%
        45%
        24.4
        DRR171782_2
        33.0%
        45%
        24.4
        DRR171783_1
        50.6%
        44%
        40.2
        DRR171783_2
        50.1%
        44%
        40.2
        DRR171786_1
        51.8%
        45%
        37.5
        DRR171786_2
        51.1%
        45%
        37.5
        DRR171791_1
        26.6%
        45%
        23.2
        DRR171791_2
        26.2%
        45%
        23.2
        DRR171793_1
        34.9%
        46%
        21.5
        DRR171793_2
        34.7%
        46%
        21.5
        DRR171796_1
        25.9%
        46%
        21.1
        DRR171796_2
        25.3%
        46%
        21.1
        DRR171798_1
        56.1%
        45%
        17.8
        DRR171798_2
        55.8%
        45%
        17.8
        DRR171801_1
        39.7%
        47%
        18.2
        DRR171801_2
        39.6%
        47%
        18.2
        DRR171802_1
        30.2%
        44%
        15.8
        DRR171802_2
        30.3%
        44%
        15.8
        DRR171807_1
        49.9%
        42%
        22.0
        DRR171807_2
        49.0%
        42%
        22.0
        DRR171810_1
        41.4%
        46%
        21.3
        DRR171810_2
        40.6%
        46%
        21.3
        DRR171812_1
        53.6%
        46%
        20.5
        DRR171812_2
        53.0%
        46%
        20.5
        DRR171813_1
        25.7%
        44%
        19.1
        DRR171813_2
        25.3%
        44%
        19.1
        DRR171816_1
        40.8%
        45%
        17.8
        DRR171816_2
        40.5%
        45%
        17.8
        DRR171817_1
        33.0%
        47%
        16.7
        DRR171817_2
        32.9%
        47%
        16.7
        DRR171821_1
        30.7%
        45%
        19.1
        DRR171821_2
        30.5%
        44%
        19.1
        DRR171825_1
        28.6%
        45%
        17.8
        DRR171825_2
        28.8%
        45%
        17.8
        DRR171829_1
        38.8%
        44%
        22.3
        DRR171829_2
        38.7%
        44%
        22.3
        DRR171833_1
        39.4%
        41%
        20.2
        DRR171833_2
        39.1%
        41%
        20.2
        DRR171834_1
        54.1%
        45%
        20.9
        DRR171834_2
        53.8%
        45%
        20.9
        DRR171838_1
        31.7%
        45%
        16.7
        DRR171838_2
        31.6%
        45%
        16.7
        DRR171840_1
        31.4%
        45%
        18.6
        DRR171840_2
        31.4%
        45%
        18.6
        DRR171842_1
        37.4%
        46%
        20.2
        DRR171842_2
        37.1%
        46%
        20.2
        DRR171843_1
        36.0%
        46%
        20.8
        DRR171843_2
        35.8%
        46%
        20.8
        DRR171845_1
        45.1%
        46%
        21.9
        DRR171845_2
        44.9%
        46%
        21.9
        DRR171849_1
        41.2%
        44%
        33.4
        DRR171849_2
        40.1%
        44%
        33.4
        DRR171850_1
        33.4%
        45%
        19.2
        DRR171850_2
        33.0%
        45%
        19.2
        DRR171852_1
        50.2%
        44%
        28.2
        DRR171852_2
        49.8%
        44%
        28.2
        DRR171855_1
        47.2%
        46%
        20.0
        DRR171855_2
        46.6%
        46%
        20.0
        DRR171856_1
        42.1%
        44%
        20.2
        DRR171856_2
        41.6%
        44%
        20.2
        DRR171862_1
        44.8%
        45%
        23.2
        DRR171862_2
        44.4%
        45%
        23.2
        DRR171863_1
        32.8%
        44%
        16.7
        DRR171863_2
        19.0%
        44%
        16.7
        DRR171864_1
        47.9%
        46%
        22.1
        DRR171864_2
        30.8%
        46%
        22.1
        DRR171865_1
        44.2%
        47%
        22.7
        DRR171865_2
        41.1%
        46%
        22.7
        DRR171870_1
        29.6%
        45%
        17.1
        DRR171870_2
        16.9%
        45%
        17.1
        DRR171872_1
        36.4%
        47%
        18.1
        DRR171872_2
        36.1%
        47%
        18.1
        DRR171876_1
        59.8%
        45%
        23.7
        DRR171876_2
        59.5%
        45%
        23.7
        DRR171886_1
        37.5%
        46%
        22.8
        DRR171886_2
        37.4%
        46%
        22.8
        DRR171890_1
        29.6%
        45%
        19.9
        DRR171890_2
        29.5%
        45%
        19.9
        DRR171897_1
        54.1%
        45%
        39.9
        DRR171897_2
        54.1%
        45%
        39.9
        DRR171899_1
        44.9%
        51%
        17.4
        DRR171899_2
        44.5%
        51%
        17.4
        DRR171902_1
        92.4%
        47%
        8.7
        DRR171902_2
        92.1%
        46%
        8.7
        DRR171915_1
        25.9%
        47%
        25.5
        DRR171915_2
        25.6%
        47%
        25.5
        DRR171917_1
        44.0%
        44%
        29.3
        DRR171917_2
        43.7%
        44%
        29.3
        DRR171919_1
        44.8%
        44%
        20.7
        DRR171919_2
        44.4%
        44%
        20.7
        DRR171921_1
        34.5%
        46%
        33.6
        DRR171921_2
        34.1%
        46%
        33.6
        DRR171922_1
        43.4%
        45%
        26.7
        DRR171922_2
        43.0%
        45%
        26.7
        DRR171923_1
        27.8%
        47%
        25.4
        DRR171923_2
        27.5%
        47%
        25.4
        DRR171925_1
        34.7%
        45%
        28.9
        DRR171925_2
        34.5%
        45%
        28.9
        DRR171928_1
        44.6%
        43%
        21.8
        DRR171928_2
        44.4%
        43%
        21.8
        DRR171930_1
        27.9%
        48%
        27.2
        DRR171930_2
        27.8%
        48%
        27.2
        DRR171931_1
        43.8%
        43%
        26.4
        DRR171931_2
        43.8%
        43%
        26.4
        DRR171933_1
        31.2%
        46%
        33.1
        DRR171933_2
        30.8%
        46%
        33.1
        DRR171934_1
        48.3%
        45%
        23.3
        DRR171934_2
        47.9%
        45%
        23.3
        DRR171935_1
        35.5%
        45%
        26.3
        DRR171935_2
        35.2%
        45%
        26.3
        DRR171936_1
        31.2%
        44%
        17.3
        DRR171936_2
        31.0%
        44%
        17.3
        DRR171938_1
        35.8%
        45%
        25.5
        DRR171938_2
        35.2%
        45%
        25.5
        DRR171939_1
        41.1%
        43%
        25.8
        DRR171939_2
        40.2%
        43%
        25.8
        DRR171941_1
        39.0%
        49%
        20.0
        DRR171941_2
        38.8%
        49%
        20.0
        DRR171942_1
        39.7%
        47%
        16.6
        DRR171942_2
        39.9%
        46%
        16.6
        DRR171943_1
        51.5%
        46%
        26.8
        DRR171943_2
        51.1%
        46%
        26.8
        DRR171948_1
        35.9%
        46%
        22.6
        DRR171948_2
        35.8%
        46%
        22.6
        DRR171950_1
        38.2%
        44%
        26.7
        DRR171950_2
        37.7%
        44%
        26.7
        DRR171951_1
        35.5%
        45%
        26.9
        DRR171951_2
        35.0%
        45%
        26.9
        DRR171952_1
        11.4%
        45%
        7.2
        DRR171952_2
        11.4%
        45%
        7.2
        DRR171953_1
        32.5%
        43%
        32.5
        DRR171953_2
        32.6%
        43%
        32.5
        DRR171954_1
        31.2%
        45%
        30.0
        DRR171954_2
        31.3%
        45%
        30.0
        DRR171957_1
        29.1%
        44%
        22.2
        DRR171957_2
        28.9%
        44%
        22.2
        DRR171959_1
        29.1%
        45%
        25.8
        DRR171959_2
        28.8%
        45%
        25.8
        DRR171964_1
        33.2%
        44%
        28.7
        DRR171964_2
        32.4%
        44%
        28.7
        DRR171965_1
        47.5%
        38%
        23.2
        DRR171965_2
        46.6%
        38%
        23.2
        DRR173016_1
        30.4%
        45%
        16.4
        DRR173016_2
        30.2%
        45%
        16.4
        DRR173017_1
        55.2%
        45%
        15.5
        DRR173017_2
        55.1%
        45%
        15.5

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Counts

        Sequence counts for each sample. Duplicate read counts are an estimate only.

        This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).

        You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        loading..

        Sequence Quality Histograms

        The mean quality value across each base position in the read.

        To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).

        Taken from the FastQC help:

        The y-axis on the graph shows the quality scores. The higher the score, the better the base call. The background of the graph divides the y axis into very good quality calls (green), calls of reasonable quality (orange), and calls of poor quality (red). The quality of calls on most platforms will degrade as the run progresses, so it is common to see base calls falling into the orange area towards the end of a read.

        loading..

        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality.

        From the FastQC help:

        The per sequence quality score report allows you to see if a subset of your sequences have universally low quality values. It is often the case that a subset of sequences will have universally poor quality, however these should represent only a small percentage of the total sequences.

        loading..

        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called.

        To enable multiple samples to be shown in a single plot, the base composition data is shown as a heatmap. The colours represent the balance between the four bases: an even distribution should give an even muddy brown colour. Hover over the plot to see the percentage of the four bases under the cursor.

        To see the data as a line plot, as in the original FastQC graph, click on a sample track.

        From the FastQC help:

        Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.

        In a random library you would expect that there would be little to no difference between the different bases of a sequence run, so the lines in this plot should run parallel with each other. The relative amount of each base should reflect the overall amount of these bases in your genome, but in any case they should not be hugely imbalanced from each other.

        It's worth noting that some types of library will always produce biased sequence composition, normally at the start of the read. Libraries produced by priming using random hexamers (including nearly all RNA-Seq libraries) and those which were fragmented using transposases inherit an intrinsic bias in the positions at which reads start. This bias does not concern an absolute sequence, but instead provides enrichement of a number of different K-mers at the 5' end of the reads. Whilst this is a true technical bias, it isn't something which can be corrected by trimming and in most cases doesn't seem to adversely affect the downstream analysis.

        Click a sample row to see a line plot for that dataset.
        Rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.

        From the FastQC help:

        This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.

        In a normal random library you would expect to see a roughly normal distribution of GC content where the central peak corresponds to the overall GC content of the underlying genome. Since we don't know the the GC content of the genome the modal GC content is calculated from the observed data and used to build a reference distribution.

        An unusually shaped distribution could indicate a contaminated library or some other kinds of biased subset. A normal distribution which is shifted indicates some systematic bias which is independent of base position. If there is a systematic bias which creates a shifted normal distribution then this won't be flagged as an error by the module since it doesn't know what your genome's GC content should be.

        loading..

        Per Base N Content

        The percentage of base calls at each position for which an N was called.

        From the FastQC help:

        If a sequencer is unable to make a base call with sufficient confidence then it will normally substitute an N rather than a conventional base call. This graph shows the percentage of base calls at each position for which an N was called.

        It's not unusual to see a very low proportion of Ns appearing in a sequence, especially nearer the end of a sequence. However, if this proportion rises above a few percent it suggests that the analysis pipeline was unable to interpret the data well enough to make valid base calls.

        loading..

        Sequence Length Distribution

        The distribution of fragment sizes (read lengths) found. See the FastQC help

        loading..

        Sequence Duplication Levels

        The relative level of duplication found for every sequence.

        From the FastQC Help:

        In a diverse library most sequences will occur only once in the final set. A low level of duplication may indicate a very high level of coverage of the target sequence, but a high level of duplication is more likely to indicate some kind of enrichment bias (eg PCR over amplification). This graph shows the degree of duplication for every sequence in a library: the relative number of sequences with different degrees of duplication.

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        In a properly diverse library most sequences should fall into the far left of the plot in both the red and blue lines. A general level of enrichment, indicating broad oversequencing in the library will tend to flatten the lines, lowering the low end and generally raising other categories. More specific enrichments of subsets, or the presence of low complexity contaminants will tend to produce spikes towards the right of the plot.

        loading..

        Overrepresented sequences

        The total amount of overrepresented sequences found in each library.

        FastQC calculates and lists overrepresented sequences in FastQ files. It would not be possible to show this for all samples in a MultiQC report, so instead this plot shows the number of sequences categorized as over represented.

        Sometimes, a single sequence may account for a large number of reads in a dataset. To show this, the bars are split into two: the first shows the overrepresented reads that come from the single most common sequence. The second shows the total count from all remaining overrepresented sequences.

        From the FastQC Help:

        A normal high-throughput library will contain a diverse set of sequences, with no individual sequence making up a tiny fraction of the whole. Finding that a single sequence is very overrepresented in the set either means that it is highly biologically significant, or indicates that the library is contaminated, or not as diverse as you expected.

        FastQC lists all of the sequences which make up more than 0.1% of the total. To conserve memory only sequences which appear in the first 100,000 sequences are tracked to the end of the file. It is therefore possible that a sequence which is overrepresented but doesn't appear at the start of the file for some reason could be missed by this module.

        494 samples had less than 1% of reads made up of overrepresented sequences

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.

        Note that only samples with ≥ 0.1% adapter contamination are shown.

        There may be several lines per sample, as one is shown for each adapter detected in the file.

        From the FastQC Help:

        The plot shows a cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. Once a sequence has been seen in a read it is counted as being present right through to the end of the read so the percentages you see will only increase as the read length goes on.

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        Status Checks

        Status for each FastQC section showing whether results seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        FastQC assigns a status for each section of the report. These give a quick evaluation of whether the results of the analysis seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        It is important to stress that although the analysis results appear to give a pass/fail result, these evaluations must be taken in the context of what you expect from your library. A 'normal' sample as far as FastQC is concerned is random and diverse. Some experiments may be expected to produce libraries which are biased in particular ways. You should treat the summary evaluations therefore as pointers to where you should concentrate your attention and understand why your library may not look random and diverse.

        Specific guidance on how to interpret the output of each module can be found in the relevant report section, or in the FastQC help.

        In this heatmap, we summarise all of these into a single heatmap for a quick overview. Note that not all FastQC sections have plots in MultiQC reports, but all status checks are shown in this heatmap.

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        MultiQC v1.14 - Written by Phil Ewels, available on GitHub.

        This report uses HighCharts, jQuery, jQuery UI, Bootstrap, FileSaver.js and clipboard.js.